{"id":31516,"date":"2025-09-12T09:05:00","date_gmt":"2025-09-12T09:05:00","guid":{"rendered":"https:\/\/amelie-project.eu\/?post_type=publication&p=31516"},"modified":"2025-12-17T16:47:20","modified_gmt":"2025-12-17T16:47:20","slug":"criopreservacao-de-combinacoes-de-microcarregadores-de-celulas-derivadas-do-musculo-esqueletico-humano-implantaveis-para-utilizacao-em-medicina-regenerativa-clinica","status":"publish","type":"publication","link":"https:\/\/amelie-project.eu\/pt\/publicacao\/criopreservacao-de-combinacoes-de-microcarregadores-de-celulas-derivadas-do-musculo-esqueletico-humano-implantaveis-para-utilizacao-em-medicina-regenerativa-clinica\/","title":{"rendered":"Criopreserva\u00e7\u00e3o de combina\u00e7\u00f5es de c\u00e9lulas-microtransportadoras implant\u00e1veis derivadas do m\u00fasculo esquel\u00e9tico humano para utiliza\u00e7\u00e3o em medicina regenerativa cl\u00ednica"},"content":{"rendered":"

[et_pb_section fb_built=”1″ admin_label=”section” _builder_version=”4.16″ global_colors_info=”{}”][et_pb_row admin_label=”row” _builder_version=”4.21.0″ background_size=”initial” background_position=”top_left” background_repeat=”repeat” width=”100%” custom_padding=”||13px|||” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_text admin_label=”Text” _builder_version=”4.21.0″ background_size=”initial” background_position=”top_left” background_repeat=”repeat” global_colors_info=”{}”]<\/p>\n

Simitzi C, Zhang J, Marjsteiner R, Fuller B, Day RM.<\/strong><\/p>\n

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Cytotherapy 12 de setembro de 2025.<\/p>\n

Resumo<\/em><\/p>\n

Este estudo analisou se pequenos gr\u00e2nulos implant\u00e1veis que transportam c\u00e9lulas musculares humanas podem ser congelados e armazenados em seguran\u00e7a para posterior utiliza\u00e7\u00e3o m\u00e9dica. Estes gr\u00e2nulos (chamados microtransportadores) foram concebidos para transportar c\u00e9lulas saud\u00e1veis para tecidos danificados, ajudando-os a reparar-se. Normalmente, estes produtos que transportam c\u00e9lulas t\u00eam de ser fabricados frescos, o que dificulta o seu fabrico e distribui\u00e7\u00e3o. Os investigadores testaram diferentes m\u00e9todos de congela\u00e7\u00e3o e descongela\u00e7\u00e3o e verificaram que as c\u00e9lulas musculares sobreviveram bem, permaneceram ligadas \u00e0s esferas e continuaram a comportar-se normalmente ap\u00f3s a descongela\u00e7\u00e3o. As pr\u00f3prias esferas tamb\u00e9m permaneceram estruturalmente s\u00f3lidas. Isto significa que, no futuro, estes tratamentos baseados em c\u00e9lulas poderiam ser produzidos antecipadamente, armazenados numa \u201ccadeia de frio\u201d e entregues nos hospitais quando necess\u00e1rio, tornando as terapias regenerativas muito mais pr\u00e1ticas e acess\u00edveis.<\/p>\n

Resumo<\/em><\/p>\n

Objectivos gerais: As terapias de medicina regenerativa incluem constru\u00e7\u00f5es de engenharia de tecidos para restaurar a fun\u00e7\u00e3o de tecidos e \u00f3rg\u00e3os. Entre as diferentes abordagens, foram propostos microtransportadores polim\u00e9ricos implant\u00e1veis para a entrega de c\u00e9lulas dependentes de ancoragem em locais-alvo dos tecidos. As combina\u00e7\u00f5es de c\u00e9lulas-microtransportadores produzidas como medicamentos de terapia avan\u00e7ada frescos enfrentam desafios significativos em termos de fabrico e tempo de distribui\u00e7\u00e3o. No presente estudo, explor\u00e1mos a viabilidade da criopreserva\u00e7\u00e3o de combina\u00e7\u00f5es de c\u00e9lulas derivadas do m\u00fasculo esquel\u00e9tico humano (SMDC)-microcarreadores implant\u00e1veis.<\/p>\n

M\u00e9todos: Foram investigadas formula\u00e7\u00f5es crioprotectoras existentes e novas, combinadas com arrefecimento lento, juntamente com regimes de descongelamento r\u00e1pido e lento.<\/p>\n

Resultados: Sob condi\u00e7\u00f5es espec\u00edficas ap\u00f3s a criopreserva\u00e7\u00e3o e descongela\u00e7\u00e3o, a maioria das c\u00e9lulas SMDC eram vi\u00e1veis e permaneceram ligadas aos microtransportadores. Al\u00e9m disso, a capacidade das SMDCs humanas para se diferenciarem em miotubos n\u00e3o foi afetada. O processo de criopreserva\u00e7\u00e3o n\u00e3o alterou as propriedades f\u00edsico-mec\u00e2nicas dos microtransportadores, permitindo-lhes manter a sua fun\u00e7\u00e3o prim\u00e1ria de substrato celular implant\u00e1vel.<\/p>\n

Conclus\u00f5es: De um modo geral, estes resultados abrem caminho \u00e0 utiliza\u00e7\u00e3o do fornecimento de produtos da cadeia de frio para futuros estudos cl\u00ednicos com a tecnologia de microtransportadores de c\u00e9lulas implant\u00e1veis.<\/p>\n

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Aceder ao artigo completo aqui:<\/p>\n

https:\/\/www.sciencedirect.com\/science\/article\/pii\/S1465324925008424<\/a><\/p>\n

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Simitzi C, Zhang J, Marjsteiner R, Fuller B, Day RM. Cytotherapy 12th September 2025. Summary This study looked at whether tiny, implantable beads carrying human muscle cells can be safely frozen and stored for later medical use. These beads (called microcarriers) are designed to deliver healthy cells to damaged tissues to help them repair themselves. Normally, these cell-carrying products must be made fresh, which makes them difficult to manufacture and distribute. The researchers tested different freezing and thawing methods and found that the muscle cells survived well, stayed attached to the beads, and still behaved normally after thawing. The beads themselves also remained structurally sound. This means that in the future, these cell-based treatments could be produced in advance, stored in a \u201ccold chain\u201d and delivered to hospitals when needed, making regenerative therapies much more practical and accessible. Abstract Background aims: Regenerative medicine therapies include tissue-engineered constructs to restore tissue and organ function. Among the different approaches, implantable polymeric microcarriers have been proposed for delivery of anchorage-dependent cells to target tissue locations. Cell-microcarrier combinations produced as fresh advanced therapy medicinal products face significant challenges in terms of manufacturing and time distribution. In the current study, we have explored the feasibility […]<\/p>","protected":false},"featured_media":31519,"template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"\n

Charlotte Desprez, Davide Danovi, Charles H Knowles and Richard M Day.<\/p>\n\n\n\n

J. Tissue Eng. 2023;14:1\u201318.<\/p>\n\n\n\n

Abstract<\/em><\/p>\n\n\n\n

Skeletal muscle-derived cells (SMDC) hold tremendous potential for replenishing dysfunctional muscle lost due to disease or trauma. Current therapeutic usage of SMDC relies on harvesting autologous cells from muscle biopsies that are subsequently expanded in vitro before re-implantation into the patient. Heterogeneity can arise from multiple factors including quality of the starting biopsy, age and comorbidity affecting the processed SMDC. Quality attributes intended for clinical use often focus on minimum levels of myogenic cell marker expression. Such approaches do not evaluate the likelihood of SMDC to differentiate and form myofibres when implanted in vivo, which ultimately determines the likelihood of muscle regeneration. Predicting the therapeutic potency of SMDC in vitro prior to implantation is key to developing successful therapeutics in regenerative medicine and reducing implementation costs. Here, we report on the development of a novel SMDC profiling tool to examine populations of cells in vitro derived from different donors. We developed an image-based pipeline to quantify morphological features and extracted cell shape descriptors. We investigated whether these could predict heterogeneity in the formation of myotubes and correlate with the myogenic fusion index. Several of the early cell shape characteristics were found to negatively correlate with the fusion index. These included total area occupied by cells, area shape, bounding box area, compactness, equivalent diameter, minimum ferret diameter, minor axis length and perimeter of SMDC at 24 h after initiating culture. The information extracted with our approach indicates live cell imaging can detect a range of cell phenotypes based on cell-shape alone and preserving cell integrity could be used to predict propensity to form myotubes in vitro and functional tissue in vivo.<\/p>\n\n\n\n

Access the full paper here: https:\/\/pubmed.ncbi.nlm.nih.gov\/36949843\/<\/a><\/p>\n","_et_gb_content_width":"","_coblocks_attr":"","_coblocks_dimensions":"","_coblocks_responsive_height":"","_coblocks_accordion_ie_support":"","_links_to":"","_links_to_target":""},"categories":[43],"class_list":["post-31516","publication","type-publication","status-publish","has-post-thumbnail","hentry","category-publication"],"_links":{"self":[{"href":"https:\/\/amelie-project.eu\/pt\/wp-json\/wp\/v2\/publication\/31516","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/amelie-project.eu\/pt\/wp-json\/wp\/v2\/publication"}],"about":[{"href":"https:\/\/amelie-project.eu\/pt\/wp-json\/wp\/v2\/types\/publication"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/amelie-project.eu\/pt\/wp-json\/wp\/v2\/media\/31519"}],"wp:attachment":[{"href":"https:\/\/amelie-project.eu\/pt\/wp-json\/wp\/v2\/media?parent=31516"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/amelie-project.eu\/pt\/wp-json\/wp\/v2\/categories?post=31516"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}