{"id":31497,"date":"2025-04-15T15:27:46","date_gmt":"2025-04-15T15:27:46","guid":{"rendered":"https:\/\/amelie-project.eu\/?post_type=publication&p=31497"},"modified":"2025-12-17T16:46:58","modified_gmt":"2025-12-17T16:46:58","slug":"microesferas-ct-visiveis-permitem-o-rastreio-in-vivo-de-todo-o-corpo-de-estruturas-injectaveis-de-engenharia-de-tecidos","status":"publish","type":"publication","link":"https:\/\/amelie-project.eu\/pt\/publicacao\/microesferas-ct-visiveis-permitem-o-rastreio-in-vivo-de-todo-o-corpo-de-estruturas-injectaveis-de-engenharia-de-tecidos\/","title":{"rendered":"As microesferas CT-Visible permitem o rastreio in vivo de todo o corpo de andaimes de engenharia de tecidos inject\u00e1veis"},"content":{"rendered":"

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Annalisa Bettini, Peter Stephen Patrick, Richard M. Day, Daniel J. Stuckey.<\/strong><\/p>\n

Materiais avan\u00e7ados para cuidados de sa\u00fade. 2024;13(17).<\/p>\n

Resumo<\/em><\/p>\n

Este estudo desenvolveu min\u00fasculos gr\u00e2nulos semelhantes a esponjas (chamados microtransportadores) que podem transportar c\u00e9lulas terap\u00eauticas e, o que \u00e9 mais importante, podem ser vistos claramente em exames de imagiologia m\u00e9dica. Os investigadores adicionaram um material de contraste inofensivo, o sulfato de b\u00e1rio, para que os gr\u00e2nulos apare\u00e7am nos exames de tomografia computorizada (TC) depois de serem injectados no corpo. Desta forma, os m\u00e9dicos podem verificar para onde vai o material implantado, quanto tempo permanece no local e se as c\u00e9lulas transplantadas permanecem vivas. Em testes laboratoriais e em estudos com animais, as esferas eram seguras, permitiam o crescimento de c\u00e9lulas e permaneciam vis\u00edveis nos exames durante pelo menos duas semanas. As microesferas tamb\u00e9m podem ser administradas atrav\u00e9s de injec\u00e7\u00f5es minimamente invasivas, incluindo no cora\u00e7\u00e3o. Globalmente, esta tecnologia poder\u00e1 ajudar a melhorar as terapias regenerativas, assegurando que os suportes de c\u00e9lulas implantados cheguem ao local certo e a\u00ed permane\u00e7am - ao mesmo tempo que d\u00e1 aos m\u00e9dicos uma forma de monitorizar o tratamento em tempo real.<\/p>\n

Resumo<\/em><\/p>\n

A entrega e a reten\u00e7\u00e3o direcionadas s\u00e3o requisitos essenciais para produtos implant\u00e1veis de engenharia de tecidos. Os m\u00e9todos de imagiologia n\u00e3o invasivos que podem confirmar a localiza\u00e7\u00e3o, a reten\u00e7\u00e3o e a biodistribui\u00e7\u00e3o de c\u00e9lulas transplantadas ligadas a suportes de engenharia de tecidos implantados ser\u00e3o inestim\u00e1veis para a otimiza\u00e7\u00e3o e o aperfei\u00e7oamento de terapias regenerativas. Para responder a esta necessidade, um suporte de engenharia de tecidos injet\u00e1vel constitu\u00eddo por microesferas altamente porosas compat\u00edveis com o transplante de c\u00e9lulas \u00e9 modificado para conter o agente de contraste de tomografia computorizada (CT) sulfato de b\u00e1rio (BaSO4). As microesferas rastre\u00e1veis apresentam uma elevada absor\u00e7\u00e3o de raios X, com contraste que permite o rastreio de todo o corpo. As microesferas s\u00e3o celularizadas com c\u00e9lulas estaminais mesenquimais GFP+ Luciferase+ e apresentam biocompatibilidade in vitro. In vivo, as microesferas celularizadas carregadas com BaSO4 s\u00e3o introduzidas no membro posterior de ratinhos, onde permanecem vi\u00e1veis durante 14 dias. O co-registo de imagens bioluminescentes em 3D e as reconstru\u00e7\u00f5es de \u00b5CT permitem a avalia\u00e7\u00e3o do material do suporte e a co-localiza\u00e7\u00e3o das c\u00e9lulas. As microesferas rastre\u00e1veis s\u00e3o tamb\u00e9m compat\u00edveis com a entrega minimamente invasiva por injec\u00e7\u00f5es intramioc\u00e1rdicas transtor\u00e1cicas guiadas por ultra-sons em ratos. Estes resultados sugerem que as microesferas carregadas com BaSO4 podem ser utilizadas como uma nova ferramenta para otimizar as t\u00e9cnicas de entrega e acompanhar a persist\u00eancia e distribui\u00e7\u00e3o de materiais de suporte implantados. Al\u00e9m disso, as microesferas podem ser celularizadas e t\u00eam o potencial de serem desenvolvidas num produto combinado injet\u00e1vel de engenharia de tecidos para regenera\u00e7\u00e3o card\u00edaca.<\/span><\/p>\n

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Aceder ao documento completo aqui:<\/p>\n

https:\/\/advanced.onlinelibrary.wiley.com\/doi\/full\/10.1002\/adhm.202303588<\/a>\u00a0<\/p>\n

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Annalisa Bettini, Peter Stephen Patrick, Richard M. Day, Daniel J. Stuckey. Advanced Healthcare Materials. 2024;13(17). Summary This study developed tiny sponge-like beads (called microcarriers) that can carry therapeutic cells and, importantly, can be seen clearly on medical imaging scans. The researchers added a harmless contrast material, barium sulphate, so the beads show up on computed tomography (CT) scans after they are injected into the body. This makes it possible for doctors to track where the implanted material goes, how long it stays in place, and whether the transplanted cells remain alive. In laboratory tests and in animal studies, the beads were safe, allowed cells to grow on them, and stayed visible on scans for at least two weeks. The microspheres could also be delivered through minimally invasive injections, including into the heart. Overall, this technology could help improve regenerative therapies by ensuring that implanted cell-carrying scaffolds reach the right location and stay there \u2013 while giving doctors a way to monitor the treatment in real time. Abstract Targeted delivery and retention are essential requirements for implantable tissue-engineered products. Non-invasive imaging methods that can confirm location, retention, and biodistribution of transplanted cells attached to implanted tissue engineering scaffolds will be invaluable […]<\/p>","protected":false},"featured_media":31499,"template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"\n

Charlotte Desprez, Davide Danovi, Charles H Knowles and Richard M Day.<\/p>\n\n\n\n

J. Tissue Eng. 2023;14:1\u201318.<\/p>\n\n\n\n

Abstract<\/em><\/p>\n\n\n\n

Skeletal muscle-derived cells (SMDC) hold tremendous potential for replenishing dysfunctional muscle lost due to disease or trauma. Current therapeutic usage of SMDC relies on harvesting autologous cells from muscle biopsies that are subsequently expanded in vitro before re-implantation into the patient. Heterogeneity can arise from multiple factors including quality of the starting biopsy, age and comorbidity affecting the processed SMDC. Quality attributes intended for clinical use often focus on minimum levels of myogenic cell marker expression. Such approaches do not evaluate the likelihood of SMDC to differentiate and form myofibres when implanted in vivo, which ultimately determines the likelihood of muscle regeneration. Predicting the therapeutic potency of SMDC in vitro prior to implantation is key to developing successful therapeutics in regenerative medicine and reducing implementation costs. Here, we report on the development of a novel SMDC profiling tool to examine populations of cells in vitro derived from different donors. We developed an image-based pipeline to quantify morphological features and extracted cell shape descriptors. We investigated whether these could predict heterogeneity in the formation of myotubes and correlate with the myogenic fusion index. Several of the early cell shape characteristics were found to negatively correlate with the fusion index. These included total area occupied by cells, area shape, bounding box area, compactness, equivalent diameter, minimum ferret diameter, minor axis length and perimeter of SMDC at 24 h after initiating culture. The information extracted with our approach indicates live cell imaging can detect a range of cell phenotypes based on cell-shape alone and preserving cell integrity could be used to predict propensity to form myotubes in vitro and functional tissue in vivo.<\/p>\n\n\n\n

Access the full paper here: https:\/\/pubmed.ncbi.nlm.nih.gov\/36949843\/<\/a><\/p>\n","_et_gb_content_width":"","_coblocks_attr":"","_coblocks_dimensions":"","_coblocks_responsive_height":"","_coblocks_accordion_ie_support":"","_links_to":"","_links_to_target":""},"categories":[43],"class_list":["post-31497","publication","type-publication","status-publish","has-post-thumbnail","hentry","category-publication"],"_links":{"self":[{"href":"https:\/\/amelie-project.eu\/pt\/wp-json\/wp\/v2\/publication\/31497","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/amelie-project.eu\/pt\/wp-json\/wp\/v2\/publication"}],"about":[{"href":"https:\/\/amelie-project.eu\/pt\/wp-json\/wp\/v2\/types\/publication"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/amelie-project.eu\/pt\/wp-json\/wp\/v2\/media\/31499"}],"wp:attachment":[{"href":"https:\/\/amelie-project.eu\/pt\/wp-json\/wp\/v2\/media?parent=31497"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/amelie-project.eu\/pt\/wp-json\/wp\/v2\/categories?post=31497"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}