{"id":29994,"date":"2023-09-10T09:09:36","date_gmt":"2023-09-10T09:09:36","guid":{"rendered":"https:\/\/amelie-project.eu\/?post_type=category-publication&p=29994"},"modified":"2024-02-26T16:22:58","modified_gmt":"2024-02-26T16:22:58","slug":"publicacao-3","status":"publish","type":"publication","link":"https:\/\/amelie-project.eu\/pt\/publicacao\/publicacao-3\/","title":{"rendered":"Considera\u00e7\u00f5es sobre o bioprocessamento para o fabrico de c\u00e9lulas terap\u00eauticas do m\u00fasculo esquel\u00e9tico e do m\u00fasculo liso"},"content":{"rendered":"

[et_pb_section fb_built=”1″ _builder_version=”4.16″ global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_row _builder_version=”4.21.0″ background_size=”initial” background_position=”top_left” background_repeat=”repeat” width=”100%” global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_column type=”4_4″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||” theme_builder_area=”post_content”][et_pb_text _builder_version=”4.21.0″ background_size=”initial” background_position=”top_left” background_repeat=”repeat” global_colors_info=”{}” theme_builder_area=”post_content”]<\/p>\n

Teresa Franchi-Mendes, Mar\u00edlia Silva, Ana Lu\u00edsa Cartaxo, Ana Fernandes-Platzgummer, Joaquim M S Cabral e Cl\u00e1udia L da Silva.<\/strong><\/p>\n

Bioengenharia. 2023;10(9):1067.<\/p>\n

As abordagens de engenharia de tecidos no contexto muscular representam um campo emergente promissor para responder aos actuais desafios terap\u00eauticos relacionados com as m\u00faltiplas condi\u00e7\u00f5es patol\u00f3gicas que afectam os compartimentos musculares, quer o m\u00fasculo esquel\u00e9tico quer o m\u00fasculo liso, respons\u00e1veis pela contra\u00e7\u00e3o involunt\u00e1ria e volunt\u00e1ria, respetivamente. Nesta revis\u00e3o, s\u00e3o abordadas v\u00e1rias carater\u00edsticas e par\u00e2metros envolvidos no bioprocessamento de c\u00e9lulas musculares. O processo de isolamento celular \u00e9 descrito, dependendo do tipo de tecido (m\u00fasculo liso ou esquel\u00e9tico), seguido da descri\u00e7\u00e3o dos desafios que envolvem a utiliza\u00e7\u00e3o de tecido de dadores adultos e das estrat\u00e9gias para ultrapassar os obst\u00e1culos \u00e0 obten\u00e7\u00e3o de n\u00fameros de c\u00e9lulas relevantes para uma aplica\u00e7\u00e3o cl\u00ednica. Especificamente, destaca-se a utiliza\u00e7\u00e3o de c\u00e9lulas estaminais\/progenitoras como fonte de c\u00e9lulas do m\u00fasculo liso e esquel\u00e9tico para o desenvolvimento de um produto celular capaz de manter a identidade e a funcionalidade da c\u00e9lula-alvo. Al\u00e9m disso, tendo em conta a necessidade de um bioprocesso robusto e rent\u00e1vel para o fabrico de c\u00e9lulas, \u00e9 tamb\u00e9m abordada a combina\u00e7\u00e3o de c\u00e9lulas musculares com biomateriais e a necessidade de aumento de escala com vista a aplica\u00e7\u00f5es cl\u00ednicas.<\/p>\n

<\/p>\n

Aceder ao documento completo aqui:<\/p>\n

<\/p>\n

https:\/\/pubmed.ncbi.nlm.nih.gov\/37760170\/<\/a><\/p>\n

<\/p>\n

[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section]<\/p>","protected":false},"excerpt":{"rendered":"

Teresa Franchi-Mendes, Mar\u00edlia Silva, Ana Lu\u00edsa Cartaxo, Ana Fernandes-Platzgummer, Joaquim M S Cabral and Cl\u00e1udia L da Silva. Bioengineering. 2023;10(9):1067. Tissue engineering approaches within the muscle context represent a promising emerging field to address the current therapeutic challenges related with multiple pathological conditions affecting the muscle compartments, either skeletal muscle or smooth muscle, responsible for involuntary and voluntary contraction, respectively. In this review, several features and parameters involved in the bioprocessing of muscle cells are addressed. The cell isolation process is depicted, depending on the type of tissue (smooth or skeletal muscle), followed by the description of the challenges involving the use of adult donor tissue and the strategies to overcome the hurdles of reaching relevant cell numbers towards a clinical application. Specifically, the use of stem\/progenitor cells is highlighted as a source for smooth and skeletal muscle cells towards the development of a cellular product able to maintain the target cell\u2019s identity and functionality. Moreover, taking into account the need for a robust and cost-effective bioprocess for cell manufacturing, the combination of muscle cells with biomaterials and the need for scale-up envisioning clinical applications are also approached. Access the full paper here: https:\/\/pubmed.ncbi.nlm.nih.gov\/37760170\/<\/p>","protected":false},"featured_media":31459,"template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"\n

Teresa Franchi-Mendes, Mar\u00edlia Silva, Ana Lu\u00edsa Cartaxo, Ana Fernandes-Platzgummer, Joaquim M S Cabral and Cl\u00e1udia L da Silva.
Bioengineering. 2023;10(9):1067.<\/strong><\/p>\n\n\n\n

Tissue engineering approaches within the muscle context represent a promising emerging field to address the current therapeutic challenges related with multiple pathological conditions affecting the muscle compartments, either skeletal muscle or smooth muscle, responsible for involuntary and voluntary contraction, respectively. In this review, several features and parameters involved in the bioprocessing of muscle cells are addressed. The cell isolation process is depicted, depending on the type of tissue (smooth or skeletal muscle), followed by the description of the challenges involving the use of adult donor tissue and the strategies to overcome the hurdles of reaching relevant cell numbers towards a clinical application. Specifically, the use of stem\/progenitor cells is highlighted as a source for smooth and skeletal muscle cells towards the development of a cellular product able to maintain the target cell\u2019s identity and functionality. Moreover, taking into account the need for a robust and cost-effective bioprocess for cell manufacturing, the combination of muscle cells with biomaterials and the need for scale-up envisioning clinical applications are also approached.<\/p>\n\n\n\n

Access the full paper here:\u00a0<\/p>\n\n\n\n

https:\/\/pubmed.ncbi.nlm.nih.gov\/37760170\/<\/a><\/p>\n","_et_gb_content_width":"","_coblocks_attr":"","_coblocks_dimensions":"","_coblocks_responsive_height":"","_coblocks_accordion_ie_support":"","_links_to":"","_links_to_target":""},"categories":[43],"class_list":["post-29994","publication","type-publication","status-publish","has-post-thumbnail","hentry","category-publication"],"_links":{"self":[{"href":"https:\/\/amelie-project.eu\/pt\/wp-json\/wp\/v2\/publication\/29994","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/amelie-project.eu\/pt\/wp-json\/wp\/v2\/publication"}],"about":[{"href":"https:\/\/amelie-project.eu\/pt\/wp-json\/wp\/v2\/types\/publication"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/amelie-project.eu\/pt\/wp-json\/wp\/v2\/media\/31459"}],"wp:attachment":[{"href":"https:\/\/amelie-project.eu\/pt\/wp-json\/wp\/v2\/media?parent=29994"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/amelie-project.eu\/pt\/wp-json\/wp\/v2\/categories?post=29994"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}