{"id":29993,"date":"2023-12-29T09:09:34","date_gmt":"2023-12-29T09:09:34","guid":{"rendered":"https:\/\/amelie-project.eu\/?post_type=category-publication&p=29993"},"modified":"2024-02-26T16:21:43","modified_gmt":"2024-02-26T16:21:43","slug":"publicacao-2","status":"publish","type":"publication","link":"https:\/\/amelie-project.eu\/pt\/publicacao\/publicacao-2\/","title":{"rendered":"Desenvolvimento de um produto de engenharia de tecidos com microtransportadores celulares para repara\u00e7\u00e3o muscular utilizando um sistema de biorreactor"},"content":{"rendered":"[et_pb_section fb_built=”1″ admin_label=”section” _builder_version=”4.16″ global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_row admin_label=”row” _builder_version=”4.21.0″ background_size=”initial” background_position=”top_left” background_repeat=”repeat” hover_enabled=”0″ global_colors_info=”{}” theme_builder_area=”post_content” width=”100%” sticky_enabled=”0″][et_pb_column type=”4_4″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||” theme_builder_area=”post_content”][et_pb_text admin_label=”Text” _builder_version=”4.21.0″ background_size=”initial” background_position=”top_left” background_repeat=”repeat” hover_enabled=”0″ global_colors_info=”{}” theme_builder_area=”post_content” sticky_enabled=”0″]
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Ana Lu\u00edsa Cartaxo, Ana Fernandes-Platzgummer, Carlos A V Rodrigues, Ana M Melo, Katja Tecklenburg, Eva Margreiter, Richard M Day, Cl\u00e1udia L da Silva, e Joaquim M S Cabral.<\/strong>
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Tissue Eng Part C Methods. 2023;29(12):583-595.<\/p>\n

A incontin\u00eancia fecal, embora n\u00e3o ponha em risco a vida, tem um grande impacto na economia e na qualidade de vida dos doentes. At\u00e9 \u00e0 data, os tratamentos dispon\u00edveis baseiam-se em abordagens cir\u00fargicas e n\u00e3o cir\u00fargicas. Estes podem ir desde altera\u00e7\u00f5es na dieta, ao treino intestinal ou \u00e0 estimula\u00e7\u00e3o do nervo sacral, mas nenhum deles oferece uma solu\u00e7\u00e3o a longo prazo. Est\u00e3o a surgir novas terapias baseadas na medicina regenerativa, que visam regenerar o m\u00fasculo do esf\u00edncter e restaurar a contin\u00eancia. Normalmente, estas consistem na administra\u00e7\u00e3o de uma suspens\u00e3o de c\u00e9lulas musculares esquel\u00e9ticas expandidas (SkMDCs) no local danificado. No entanto, esta estrat\u00e9gia resulta frequentemente numa viabilidade celular reduzida devido \u00e0 necessidade de colheita de c\u00e9lulas da plataforma de expans\u00e3o, bem como \u00e0 utiliza\u00e7\u00e3o n\u00e3o nativa de uma suspens\u00e3o de c\u00e9lulas para administrar as c\u00e9lulas dependentes de ancoragem. Neste estudo, propomos a prova de conceito para o bioprocessamento de um novo m\u00e9todo de entrega de c\u00e9lulas para o tratamento da incontin\u00eancia fecal, obtido por um processo escal\u00e1vel em duas etapas. Em primeiro lugar, as SkMDCs isoladas dos doentes foram expandidas utilizando sistemas de cultura est\u00e1tica planar. Em segundo lugar, utilizando um bioreactor PBS-MINI Vertical-Wheel\u00ae de utiliza\u00e7\u00e3o \u00fanica, os SkMDCs expandidos foram combinados com microtransportadores biocompat\u00edveis e biodegrad\u00e1veis (ou seja, diretamente implant\u00e1veis) de \u00e1cido poli(l\u00e1tico-co-glic\u00f3lico) preparados por separa\u00e7\u00e3o de fases induzida termicamente. Este processo permitiu uma efici\u00eancia de at\u00e9 80% dos SkMDCs para se ligarem aos microtransportadores. \u00c9 importante notar que as SkMDCs foram vi\u00e1veis durante todo o processo e mantiveram as suas carater\u00edsticas miog\u00e9nicas (por exemplo, express\u00e3o do marcador CD56) ap\u00f3s a ades\u00e3o e cultura nos microtransportadores. Quando os microtransportadores contendo SkMDC foram colocados numa placa de cultura, as c\u00e9lulas foram capazes de migrar dos microtransportadores para a superf\u00edcie de cultura e diferenciar-se em miotubos multinucleados, o que real\u00e7a o seu potencial para regenerar o m\u00fasculo do esf\u00edncter danificado ap\u00f3s a administra\u00e7\u00e3o no doente. Globalmente, este estudo prop\u00f5e um m\u00e9todo inovador para fixar SkMDCs a microtransportadores biodegrad\u00e1veis, o que pode proporcionar um novo tratamento para a incontin\u00eancia fecal.<\/p>\n

Aceder ao documento completo aqui:\u00a0<\/span><\/p>\n

https:\/\/pubmed.ncbi.nlm.nih.gov\/37842845\/<\/a><\/p>\n<\/div>\n<\/div>\n

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Ana Lu\u00edsa Cartaxo, Ana Fernandes-Platzgummer, Carlos A V Rodrigues, Ana M Melo, Katja Tecklenburg, Eva Margreiter, Richard M Day, Cl\u00e1udia L da Silva, and Joaquim M S Cabral. Tissue Eng Part C Methods. 2023;29(12):583\u2013595. Fecal incontinence, although not life-threatening, has a high impact on the economy and patient quality of life. So far, available treatments are based on both surgical and nonsurgical approaches. These can range from changes in diet, to bowel training, or sacral nerve stimulation, but none of which provides a long-term solution. New regenerative medicine-based therapies are emerging, which aim at regenerating the sphincter muscle and restoring continence. Usually, these consist of the administration of a suspension of expanded skeletal-derived muscle cells (SkMDCs) to the damaged site. However, this strategy often results in a reduced cell viability due to the need for cell harvesting from the expansion platform, as well as the non-native use of a cell suspension to deliver the anchorage-dependent cells. In this study, we propose the proof-of-concept for the bioprocessing of a new cell delivery method for the treatment of fecal incontinence, obtained by a scalable two-step process. First, patient-isolated SkMDCs were expanded using planar static culture systems. Second, by using a single-use PBS-MINI […]<\/p>","protected":false},"featured_media":31458,"template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"\n

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