{"id":29992,"date":"2023-01-14T09:09:30","date_gmt":"2023-01-14T09:09:30","guid":{"rendered":"https:\/\/amelie-project.eu\/?post_type=category-publication&p=29992"},"modified":"2024-02-26T16:21:23","modified_gmt":"2024-02-26T16:21:23","slug":"publicacao-1","status":"publish","type":"publication","link":"https:\/\/amelie-project.eu\/pt\/publicacao\/publicacao-1\/","title":{"rendered":"Carater\u00edsticas da forma celular das c\u00e9lulas do m\u00fasculo esquel\u00e9tico humano como fator de previs\u00e3o da compet\u00eancia miog\u00e9nica: Um novo paradigma para uma terapia celular de precis\u00e3o"},"content":{"rendered":"[et_pb_section fb_built=”1″ admin_label=”section” _builder_version=”4.16″ global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_row admin_label=”row” _builder_version=”4.21.0″ background_size=”initial” background_position=”top_left” background_repeat=”repeat” hover_enabled=”0″ global_colors_info=”{}” theme_builder_area=”post_content” width=”100%” sticky_enabled=”0″][et_pb_column type=”4_4″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||” theme_builder_area=”post_content”][et_pb_text admin_label=”Text” _builder_version=”4.21.0″ background_size=”initial” background_position=”top_left” background_repeat=”repeat” hover_enabled=”0″ global_colors_info=”{}” theme_builder_area=”post_content” sticky_enabled=”0″]

Charlotte Desprez, Davide Danovi, Charles H Knowles e Richard M Day.<\/strong><\/p>\n

J. Tissue Eng. 2023;14:1-18.<\/p>\n

Resumo<\/em><\/p>\n

As c\u00e9lulas derivadas do m\u00fasculo esquel\u00e9tico (SMDC) t\u00eam um enorme potencial para repor o m\u00fasculo disfuncional perdido devido a doen\u00e7a ou traumatismo. A utiliza\u00e7\u00e3o terap\u00eautica atual das SMDC baseia-se na colheita de c\u00e9lulas aut\u00f3logas a partir de bi\u00f3psias musculares que s\u00e3o subsequentemente expandidas in vitro antes de serem reimplantadas no doente. A heterogeneidade pode resultar de m\u00faltiplos factores, incluindo a qualidade da bi\u00f3psia inicial, a idade e a comorbilidade que afectam as SMDC processadas. Os atributos de qualidade destinados a utiliza\u00e7\u00e3o cl\u00ednica centram-se frequentemente em n\u00edveis m\u00ednimos de express\u00e3o de marcadores de c\u00e9lulas miog\u00e9nicas. Estas abordagens n\u00e3o avaliam a probabilidade de as SMDC se diferenciarem e formarem miofibras quando implantadas in vivo, o que, em \u00faltima an\u00e1lise, determina a probabilidade de regenera\u00e7\u00e3o muscular. A previs\u00e3o da pot\u00eancia terap\u00eautica das SMDC in vitro antes da implanta\u00e7\u00e3o \u00e9 fundamental para o desenvolvimento de terap\u00eauticas bem sucedidas na medicina regenerativa e para a redu\u00e7\u00e3o dos custos de implementa\u00e7\u00e3o. Neste artigo, apresentamos o desenvolvimento de uma nova ferramenta de carateriza\u00e7\u00e3o de SMDC para examinar popula\u00e7\u00f5es de c\u00e9lulas in vitro derivadas de diferentes dadores. Desenvolvemos um pipeline baseado em imagens para quantificar as carater\u00edsticas morfol\u00f3gicas e extra\u00edmos descritores da forma das c\u00e9lulas. Investig\u00e1mos se estes poderiam prever a heterogeneidade na forma\u00e7\u00e3o de miotubos e correlacionar-se com o \u00edndice de fus\u00e3o miog\u00e9nica. Verificou-se que v\u00e1rias das carater\u00edsticas iniciais da forma das c\u00e9lulas se correlacionavam negativamente com o \u00edndice de fus\u00e3o. Estas inclu\u00edam a \u00e1rea total ocupada pelas c\u00e9lulas, a forma da \u00e1rea, a \u00e1rea da caixa delimitadora, a compacta\u00e7\u00e3o, o di\u00e2metro equivalente, o di\u00e2metro m\u00ednimo de ferret, o comprimento do eixo menor e o per\u00edmetro do SMDC 24 horas ap\u00f3s o in\u00edcio da cultura. A informa\u00e7\u00e3o extra\u00edda com a nossa abordagem indica que a imagiologia de c\u00e9lulas vivas pode detetar uma gama de fen\u00f3tipos celulares com base apenas na forma das c\u00e9lulas e que a preserva\u00e7\u00e3o da integridade celular pode ser utilizada para prever a propens\u00e3o para formar miotubos in vitro e tecido funcional in vivo.<\/p>\n

<\/p>\n

Aceder ao documento completo aqui:<\/p>\n

https:\/\/pubmed.ncbi.nlm.nih.gov\/36949843\/<\/a><\/p>\n

<\/p>[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section]","protected":false},"excerpt":{"rendered":"

Charlotte Desprez, Davide Danovi, Charles H Knowles and Richard M Day. J. Tissue Eng. 2023;14:1\u201318. Abstract Skeletal muscle-derived cells (SMDC) hold tremendous potential for replenishing dysfunctional muscle lost due to disease or trauma. Current therapeutic usage of SMDC relies on harvesting autologous cells from muscle biopsies that are subsequently expanded in vitro before re-implantation into the patient. Heterogeneity can arise from multiple factors including quality of the starting biopsy, age and comorbidity affecting the processed SMDC. Quality attributes intended for clinical use often focus on minimum levels of myogenic cell marker expression. Such approaches do not evaluate the likelihood of SMDC to differentiate and form myofibres when implanted in vivo, which ultimately determines the likelihood of muscle regeneration. Predicting the therapeutic potency of SMDC in vitro prior to implantation is key to developing successful therapeutics in regenerative medicine and reducing implementation costs. Here, we report on the development of a novel SMDC profiling tool to examine populations of cells in vitro derived from different donors. We developed an image-based pipeline to quantify morphological features and extracted cell shape descriptors. We investigated whether these could predict heterogeneity in the formation of myotubes and correlate with the myogenic fusion index. Several of […]<\/p>","protected":false},"featured_media":31457,"template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"\n

Charlotte Desprez, Davide Danovi, Charles H Knowles and Richard M Day.<\/p>\n\n\n\n

J. Tissue Eng. 2023;14:1\u201318.<\/p>\n\n\n\n

Abstract<\/em><\/p>\n\n\n\n

Skeletal muscle-derived cells (SMDC) hold tremendous potential for replenishing dysfunctional muscle lost due to disease or trauma. Current therapeutic usage of SMDC relies on harvesting autologous cells from muscle biopsies that are subsequently expanded in vitro before re-implantation into the patient. Heterogeneity can arise from multiple factors including quality of the starting biopsy, age and comorbidity affecting the processed SMDC. Quality attributes intended for clinical use often focus on minimum levels of myogenic cell marker expression. Such approaches do not evaluate the likelihood of SMDC to differentiate and form myofibres when implanted in vivo, which ultimately determines the likelihood of muscle regeneration. Predicting the therapeutic potency of SMDC in vitro prior to implantation is key to developing successful therapeutics in regenerative medicine and reducing implementation costs. Here, we report on the development of a novel SMDC profiling tool to examine populations of cells in vitro derived from different donors. We developed an image-based pipeline to quantify morphological features and extracted cell shape descriptors. We investigated whether these could predict heterogeneity in the formation of myotubes and correlate with the myogenic fusion index. Several of the early cell shape characteristics were found to negatively correlate with the fusion index. These included total area occupied by cells, area shape, bounding box area, compactness, equivalent diameter, minimum ferret diameter, minor axis length and perimeter of SMDC at 24 h after initiating culture. The information extracted with our approach indicates live cell imaging can detect a range of cell phenotypes based on cell-shape alone and preserving cell integrity could be used to predict propensity to form myotubes in vitro and functional tissue in vivo.<\/p>\n\n\n\n

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