{"id":29992,"date":"2023-01-14T09:09:30","date_gmt":"2023-01-14T09:09:30","guid":{"rendered":"https:\/\/amelie-project.eu\/?post_type=category-publication&p=29992"},"modified":"2024-02-26T16:21:23","modified_gmt":"2024-02-26T16:21:23","slug":"pubblicazione-1","status":"publish","type":"publication","link":"https:\/\/amelie-project.eu\/it\/pubblicazione\/pubblicazione-1\/","title":{"rendered":"Caratteristiche della forma cellulare delle cellule muscolari scheletriche umane come predittore della competenza miogenica: Un nuovo paradigma verso la terapia cellulare di precisione"},"content":{"rendered":"[et_pb_section fb_built=”1″ admin_label=”section” _builder_version=”4.16″ global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_row admin_label=”row” _builder_version=”4.21.0″ background_size=”initial” background_position=”top_left” background_repeat=”repeat” hover_enabled=”0″ global_colors_info=”{}” theme_builder_area=”post_content” width=”100%” sticky_enabled=”0″][et_pb_column type=”4_4″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||” theme_builder_area=”post_content”][et_pb_text admin_label=”Text” _builder_version=”4.21.0″ background_size=”initial” background_position=”top_left” background_repeat=”repeat” hover_enabled=”0″ global_colors_info=”{}” theme_builder_area=”post_content” sticky_enabled=”0″]

Charlotte Desprez, Davide Danovi, Charles H Knowles e Richard M Day.<\/strong><\/p>\n

J. Tissue Eng. 2023;14:1-18.<\/p>\n

Astratto<\/em><\/p>\n

Le cellule derivate dal muscolo scheletrico (SMDC) hanno un enorme potenziale per reintegrare i muscoli disfunzionali persi a causa di malattie o traumi. L'attuale uso terapeutico delle SMDC si basa sulla raccolta di cellule autologhe da biopsie muscolari che vengono successivamente espanse in vitro prima di essere reimpiantate nel paziente. L'eterogeneit\u00e0 pu\u00f2 derivare da molteplici fattori, tra cui la qualit\u00e0 della biopsia di partenza, l'et\u00e0 e le comorbidit\u00e0 che influenzano le SMDC trattate. Gli attributi di qualit\u00e0 destinati all'uso clinico si concentrano spesso sui livelli minimi di espressione dei marcatori cellulari miogenici. Questi approcci non valutano la probabilit\u00e0 che le SMDC si differenzino e formino miofibre quando vengono impiantate in vivo, il che determina in ultima analisi la probabilit\u00e0 di rigenerazione muscolare. Prevedere la potenza terapeutica delle SMDC in vitro prima dell'impianto \u00e8 fondamentale per sviluppare terapie di successo nella medicina rigenerativa e ridurre i costi di implementazione. Qui riportiamo lo sviluppo di un nuovo strumento di profilazione delle SMDC per esaminare le popolazioni di cellule in vitro derivate da donatori diversi. Abbiamo sviluppato una pipeline basata su immagini per quantificare le caratteristiche morfologiche ed estrarre descrittori di forma cellulare. Abbiamo studiato se questi potessero prevedere l'eterogeneit\u00e0 nella formazione dei miotubi e correlarsi con l'indice di fusione miogenica. Diverse caratteristiche della forma cellulare iniziale sono risultate correlate negativamente con l'indice di fusione. Queste includono l'area totale occupata dalle cellule, la forma dell'area, l'area della scatola di confine, la compattezza, il diametro equivalente, il diametro minimo del furetto, la lunghezza dell'asse minore e il perimetro delle SMDC a 24 ore dall'inizio della coltura. Le informazioni estratte con il nostro approccio indicano che l'imaging di cellule vive pu\u00f2 rilevare una serie di fenotipi cellulari basati solo sulla forma delle cellule e che la conservazione dell'integrit\u00e0 cellulare potrebbe essere utilizzata per prevedere la propensione a formare miotubi in vitro e tessuti funzionali in vivo.<\/p>\n

<\/p>\n

Accedere al documento completo qui:<\/p>\n

https:\/\/pubmed.ncbi.nlm.nih.gov\/36949843\/<\/a><\/p>\n

<\/p>[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section]","protected":false},"excerpt":{"rendered":"

Charlotte Desprez, Davide Danovi, Charles H Knowles and Richard M Day. J. Tissue Eng. 2023;14:1\u201318. Abstract Skeletal muscle-derived cells (SMDC) hold tremendous potential for replenishing dysfunctional muscle lost due to disease or trauma. Current therapeutic usage of SMDC relies on harvesting autologous cells from muscle biopsies that are subsequently expanded in vitro before re-implantation into the patient. Heterogeneity can arise from multiple factors including quality of the starting biopsy, age and comorbidity affecting the processed SMDC. Quality attributes intended for clinical use often focus on minimum levels of myogenic cell marker expression. Such approaches do not evaluate the likelihood of SMDC to differentiate and form myofibres when implanted in vivo, which ultimately determines the likelihood of muscle regeneration. Predicting the therapeutic potency of SMDC in vitro prior to implantation is key to developing successful therapeutics in regenerative medicine and reducing implementation costs. Here, we report on the development of a novel SMDC profiling tool to examine populations of cells in vitro derived from different donors. We developed an image-based pipeline to quantify morphological features and extracted cell shape descriptors. We investigated whether these could predict heterogeneity in the formation of myotubes and correlate with the myogenic fusion index. Several of […]<\/p>","protected":false},"featured_media":31457,"template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"\n

Charlotte Desprez, Davide Danovi, Charles H Knowles and Richard M Day.<\/p>\n\n\n\n

J. Tissue Eng. 2023;14:1\u201318.<\/p>\n\n\n\n

Abstract<\/em><\/p>\n\n\n\n

Skeletal muscle-derived cells (SMDC) hold tremendous potential for replenishing dysfunctional muscle lost due to disease or trauma. Current therapeutic usage of SMDC relies on harvesting autologous cells from muscle biopsies that are subsequently expanded in vitro before re-implantation into the patient. Heterogeneity can arise from multiple factors including quality of the starting biopsy, age and comorbidity affecting the processed SMDC. Quality attributes intended for clinical use often focus on minimum levels of myogenic cell marker expression. Such approaches do not evaluate the likelihood of SMDC to differentiate and form myofibres when implanted in vivo, which ultimately determines the likelihood of muscle regeneration. Predicting the therapeutic potency of SMDC in vitro prior to implantation is key to developing successful therapeutics in regenerative medicine and reducing implementation costs. Here, we report on the development of a novel SMDC profiling tool to examine populations of cells in vitro derived from different donors. We developed an image-based pipeline to quantify morphological features and extracted cell shape descriptors. We investigated whether these could predict heterogeneity in the formation of myotubes and correlate with the myogenic fusion index. Several of the early cell shape characteristics were found to negatively correlate with the fusion index. These included total area occupied by cells, area shape, bounding box area, compactness, equivalent diameter, minimum ferret diameter, minor axis length and perimeter of SMDC at 24 h after initiating culture. The information extracted with our approach indicates live cell imaging can detect a range of cell phenotypes based on cell-shape alone and preserving cell integrity could be used to predict propensity to form myotubes in vitro and functional tissue in vivo.<\/p>\n\n\n\n

Access the full paper here: https:\/\/pubmed.ncbi.nlm.nih.gov\/36949843\/<\/a><\/p>\n","_et_gb_content_width":"","_coblocks_attr":"","_coblocks_dimensions":"","_coblocks_responsive_height":"","_coblocks_accordion_ie_support":"","_links_to":"","_links_to_target":""},"categories":[43],"class_list":["post-29992","publication","type-publication","status-publish","has-post-thumbnail","hentry","category-publication"],"_links":{"self":[{"href":"https:\/\/amelie-project.eu\/it\/wp-json\/wp\/v2\/publication\/29992","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/amelie-project.eu\/it\/wp-json\/wp\/v2\/publication"}],"about":[{"href":"https:\/\/amelie-project.eu\/it\/wp-json\/wp\/v2\/types\/publication"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/amelie-project.eu\/it\/wp-json\/wp\/v2\/media\/31457"}],"wp:attachment":[{"href":"https:\/\/amelie-project.eu\/it\/wp-json\/wp\/v2\/media?parent=29992"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/amelie-project.eu\/it\/wp-json\/wp\/v2\/categories?post=29992"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}