{"id":29993,"date":"2023-12-29T09:09:34","date_gmt":"2023-12-29T09:09:34","guid":{"rendered":"https:\/\/amelie-project.eu\/?post_type=category-publication&p=29993"},"modified":"2024-02-26T16:21:43","modified_gmt":"2024-02-26T16:21:43","slug":"publication-2","status":"publish","type":"publication","link":"https:\/\/amelie-project.eu\/fr\/publication\/publication-2\/","title":{"rendered":"D\u00e9veloppement d'un produit d'ing\u00e9nierie tissulaire \u00e0 base de cellules et de microporteurs pour la r\u00e9paration musculaire \u00e0 l'aide d'un syst\u00e8me de bior\u00e9acteur"},"content":{"rendered":"[et_pb_section fb_built=”1″ admin_label=”section” _builder_version=”4.16″ global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_row admin_label=”row” _builder_version=”4.21.0″ background_size=”initial” background_position=”top_left” background_repeat=”repeat” hover_enabled=”0″ global_colors_info=”{}” theme_builder_area=”post_content” width=”100%” sticky_enabled=”0″][et_pb_column type=”4_4″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||” theme_builder_area=”post_content”][et_pb_text admin_label=”Text” _builder_version=”4.21.0″ background_size=”initial” background_position=”top_left” background_repeat=”repeat” hover_enabled=”0″ global_colors_info=”{}” theme_builder_area=”post_content” sticky_enabled=”0″]
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Ana Lu\u00edsa Cartaxo, Ana Fernandes-Platzgummer, Carlos A V Rodrigues, Ana M Melo, Katja Tecklenburg, Eva Margreiter, Richard M Day, Cl\u00e1udia L da Silva et Joaquim M S Cabral.<\/strong>
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Tissue Eng Part C Methods. 2023;29(12):583-595.<\/p>\n

L'incontinence f\u00e9cale, bien qu'elle ne mette pas la vie en danger, a un impact important sur l'\u00e9conomie et la qualit\u00e9 de vie des patients. Jusqu'\u00e0 pr\u00e9sent, les traitements disponibles sont bas\u00e9s sur des approches chirurgicales et non chirurgicales. Celles-ci vont de la modification du r\u00e9gime alimentaire \u00e0 l'entra\u00eenement \u00e0 la d\u00e9f\u00e9cation, en passant par la stimulation du nerf sacr\u00e9, mais aucune d'entre elles ne constitue une solution \u00e0 long terme. De nouvelles th\u00e9rapies bas\u00e9es sur la m\u00e9decine r\u00e9g\u00e9n\u00e9rative \u00e9mergent, qui visent \u00e0 r\u00e9g\u00e9n\u00e9rer le muscle sphincter et \u00e0 restaurer la continence. Elles consistent g\u00e9n\u00e9ralement en l'administration d'une suspension de cellules musculaires d\u00e9riv\u00e9es du squelette (SkMDC) sur le site endommag\u00e9. Cependant, cette strat\u00e9gie se traduit souvent par une viabilit\u00e9 cellulaire r\u00e9duite en raison de la n\u00e9cessit\u00e9 de pr\u00e9lever des cellules sur la plateforme d'expansion, ainsi que de l'utilisation non native d'une suspension cellulaire pour administrer les cellules d\u00e9pendantes de l'ancrage. Dans cette \u00e9tude, nous proposons la preuve de concept pour le biotraitement d'une nouvelle m\u00e9thode d'administration de cellules pour le traitement de l'incontinence f\u00e9cale, obtenue par un processus \u00e9volutif en deux \u00e9tapes. Tout d'abord, des SkMDC isol\u00e9es par le patient ont \u00e9t\u00e9 expans\u00e9es \u00e0 l'aide de syst\u00e8mes de culture statiques planaires. Ensuite, \u00e0 l'aide d'un bior\u00e9acteur PBS-MINI Vertical-Wheel\u00ae \u00e0 usage unique, les SkMDC expans\u00e9es ont \u00e9t\u00e9 associ\u00e9es \u00e0 des microporteurs biocompatibles et biod\u00e9gradables (c'est-\u00e0-dire directement implantables) d'acide poly(lactique-co-glycolique) pr\u00e9par\u00e9s par s\u00e9paration de phase induite thermiquement. Ce processus a permis aux SkMDC de se fixer sur les microporteurs avec une efficacit\u00e9 allant jusqu'\u00e0 80%. Il est important de noter que les SkMDC \u00e9taient viables pendant tout le processus et qu'elles ont conserv\u00e9 leurs caract\u00e9ristiques myog\u00e9niques (par exemple, l'expression du marqueur CD56) apr\u00e8s l'adh\u00e9sion et la culture sur les microporteurs. Lorsque les microporteurs contenant des SkMDC ont \u00e9t\u00e9 plac\u00e9s sur une bo\u00eete de culture, les cellules ont pu migrer des microporteurs vers la surface de culture et se diff\u00e9rencier en myotubes multinucl\u00e9\u00e9s, ce qui souligne leur potentiel de r\u00e9g\u00e9n\u00e9ration du muscle sphinct\u00e9rien endommag\u00e9 apr\u00e8s administration au patient. Dans l'ensemble, cette \u00e9tude propose une m\u00e9thode innovante pour fixer les SkMDC sur des microporteurs biod\u00e9gradables, ce qui peut constituer un nouveau traitement pour l'incontinence f\u00e9cale.<\/p>\n

Acc\u00e9der \u00e0 l'article complet ici :\u00a0<\/span><\/p>\n

https:\/\/pubmed.ncbi.nlm.nih.gov\/37842845\/<\/a><\/p>\n<\/div>\n<\/div>\n

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Ana Lu\u00edsa Cartaxo, Ana Fernandes-Platzgummer, Carlos A V Rodrigues, Ana M Melo, Katja Tecklenburg, Eva Margreiter, Richard M Day, Cl\u00e1udia L da Silva, and Joaquim M S Cabral. Tissue Eng Part C Methods. 2023;29(12):583\u2013595. Fecal incontinence, although not life-threatening, has a high impact on the economy and patient quality of life. So far, available treatments are based on both surgical and nonsurgical approaches. These can range from changes in diet, to bowel training, or sacral nerve stimulation, but none of which provides a long-term solution. New regenerative medicine-based therapies are emerging, which aim at regenerating the sphincter muscle and restoring continence. Usually, these consist of the administration of a suspension of expanded skeletal-derived muscle cells (SkMDCs) to the damaged site. However, this strategy often results in a reduced cell viability due to the need for cell harvesting from the expansion platform, as well as the non-native use of a cell suspension to deliver the anchorage-dependent cells. In this study, we propose the proof-of-concept for the bioprocessing of a new cell delivery method for the treatment of fecal incontinence, obtained by a scalable two-step process. First, patient-isolated SkMDCs were expanded using planar static culture systems. Second, by using a single-use PBS-MINI […]<\/p>","protected":false},"featured_media":31458,"template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"\n

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