{"id":29992,"date":"2023-01-14T09:09:30","date_gmt":"2023-01-14T09:09:30","guid":{"rendered":"https:\/\/amelie-project.eu\/?post_type=category-publication&p=29992"},"modified":"2024-02-26T16:21:23","modified_gmt":"2024-02-26T16:21:23","slug":"publication-1","status":"publish","type":"publication","link":"https:\/\/amelie-project.eu\/fr\/publication\/publication-1\/","title":{"rendered":"Caract\u00e9ristiques de la forme cellulaire des cellules musculaires squelettiques humaines en tant que pr\u00e9dicteur de la comp\u00e9tence myog\u00e9nique : Un nouveau paradigme pour une th\u00e9rapie cellulaire de pr\u00e9cision"},"content":{"rendered":"[et_pb_section fb_built=”1″ admin_label=”section” _builder_version=”4.16″ global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_row admin_label=”row” _builder_version=”4.21.0″ background_size=”initial” background_position=”top_left” background_repeat=”repeat” hover_enabled=”0″ global_colors_info=”{}” theme_builder_area=”post_content” width=”100%” sticky_enabled=”0″][et_pb_column type=”4_4″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||” theme_builder_area=”post_content”][et_pb_text admin_label=”Text” _builder_version=”4.21.0″ background_size=”initial” background_position=”top_left” background_repeat=”repeat” hover_enabled=”0″ global_colors_info=”{}” theme_builder_area=”post_content” sticky_enabled=”0″]

Charlotte Desprez, Davide Danovi, Charles H Knowles et Richard M Day.<\/strong><\/p>\n

J. Tissue Eng. 2023;14:1-18.<\/p>\n

R\u00e9sum\u00e9<\/em><\/p>\n

Les cellules d\u00e9riv\u00e9es des muscles squelettiques (CDSM) offrent un potentiel consid\u00e9rable pour reconstituer les muscles dysfonctionnels perdus \u00e0 la suite d'une maladie ou d'un traumatisme. L'utilisation th\u00e9rapeutique actuelle des CSDM repose sur le pr\u00e9l\u00e8vement de cellules autologues \u00e0 partir de biopsies musculaires qui sont ensuite d\u00e9velopp\u00e9es in vitro avant d'\u00eatre r\u00e9implant\u00e9es chez le patient. L'h\u00e9t\u00e9rog\u00e9n\u00e9it\u00e9 peut provenir de multiples facteurs, dont la qualit\u00e9 de la biopsie initiale, l'\u00e2ge et la comorbidit\u00e9, qui affectent le SMDC trait\u00e9. Les attributs de qualit\u00e9 destin\u00e9s \u00e0 un usage clinique se concentrent souvent sur des niveaux minimaux d'expression de marqueurs de cellules myog\u00e9niques. Ces approches n'\u00e9valuent pas la probabilit\u00e9 que les CSDM se diff\u00e9rencient et forment des myofibres lorsqu'ils sont implant\u00e9s in vivo, ce qui d\u00e9termine en fin de compte la probabilit\u00e9 d'une r\u00e9g\u00e9n\u00e9ration musculaire. Pr\u00e9dire le pouvoir th\u00e9rapeutique du SMDC in vitro avant l'implantation est essentiel pour d\u00e9velopper des th\u00e9rapies r\u00e9ussies en m\u00e9decine r\u00e9g\u00e9n\u00e9rative et r\u00e9duire les co\u00fbts de mise en \u0153uvre. Nous pr\u00e9sentons ici le d\u00e9veloppement d'un nouvel outil de profilage des SMDC pour examiner les populations de cellules in vitro d\u00e9riv\u00e9es de diff\u00e9rents donneurs. Nous avons d\u00e9velopp\u00e9 un pipeline bas\u00e9 sur l'image pour quantifier les caract\u00e9ristiques morphologiques et extraire des descripteurs de la forme des cellules. Nous avons cherch\u00e9 \u00e0 savoir si ces descripteurs pouvaient pr\u00e9dire l'h\u00e9t\u00e9rog\u00e9n\u00e9it\u00e9 dans la formation des myotubes et \u00eatre corr\u00e9l\u00e9s avec l'indice de fusion myog\u00e9nique. Plusieurs des caract\u00e9ristiques pr\u00e9coces de la forme des cellules se sont r\u00e9v\u00e9l\u00e9es \u00eatre en corr\u00e9lation n\u00e9gative avec l'indice de fusion. Il s'agit de la surface totale occup\u00e9e par les cellules, de la forme de la surface, de la surface de la bo\u00eete de d\u00e9limitation, de la compacit\u00e9, du diam\u00e8tre \u00e9quivalent, du diam\u00e8tre minimum du furet, de la longueur du petit axe et du p\u00e9rim\u00e8tre du SMDC \u00e0 24 heures apr\u00e8s le d\u00e9but de la culture. Les informations extraites avec notre approche indiquent que l'imagerie des cellules vivantes peut d\u00e9tecter une gamme de ph\u00e9notypes cellulaires bas\u00e9s uniquement sur la forme des cellules et que la pr\u00e9servation de l'int\u00e9grit\u00e9 cellulaire pourrait \u00eatre utilis\u00e9e pour pr\u00e9dire la propension \u00e0 former des myotubes in vitro et des tissus fonctionnels in vivo.<\/p>\n

<\/p>\n

Acc\u00e9der \u00e0 l'article complet ici :<\/p>\n

https:\/\/pubmed.ncbi.nlm.nih.gov\/36949843\/<\/a><\/p>\n

<\/p>[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section]","protected":false},"excerpt":{"rendered":"

Charlotte Desprez, Davide Danovi, Charles H Knowles and Richard M Day. J. Tissue Eng. 2023;14:1\u201318. Abstract Skeletal muscle-derived cells (SMDC) hold tremendous potential for replenishing dysfunctional muscle lost due to disease or trauma. Current therapeutic usage of SMDC relies on harvesting autologous cells from muscle biopsies that are subsequently expanded in vitro before re-implantation into the patient. Heterogeneity can arise from multiple factors including quality of the starting biopsy, age and comorbidity affecting the processed SMDC. Quality attributes intended for clinical use often focus on minimum levels of myogenic cell marker expression. Such approaches do not evaluate the likelihood of SMDC to differentiate and form myofibres when implanted in vivo, which ultimately determines the likelihood of muscle regeneration. Predicting the therapeutic potency of SMDC in vitro prior to implantation is key to developing successful therapeutics in regenerative medicine and reducing implementation costs. Here, we report on the development of a novel SMDC profiling tool to examine populations of cells in vitro derived from different donors. We developed an image-based pipeline to quantify morphological features and extracted cell shape descriptors. We investigated whether these could predict heterogeneity in the formation of myotubes and correlate with the myogenic fusion index. Several of […]<\/p>","protected":false},"featured_media":31457,"template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"\n

Charlotte Desprez, Davide Danovi, Charles H Knowles and Richard M Day.<\/p>\n\n\n\n

J. Tissue Eng. 2023;14:1\u201318.<\/p>\n\n\n\n

Abstract<\/em><\/p>\n\n\n\n

Skeletal muscle-derived cells (SMDC) hold tremendous potential for replenishing dysfunctional muscle lost due to disease or trauma. Current therapeutic usage of SMDC relies on harvesting autologous cells from muscle biopsies that are subsequently expanded in vitro before re-implantation into the patient. Heterogeneity can arise from multiple factors including quality of the starting biopsy, age and comorbidity affecting the processed SMDC. Quality attributes intended for clinical use often focus on minimum levels of myogenic cell marker expression. Such approaches do not evaluate the likelihood of SMDC to differentiate and form myofibres when implanted in vivo, which ultimately determines the likelihood of muscle regeneration. Predicting the therapeutic potency of SMDC in vitro prior to implantation is key to developing successful therapeutics in regenerative medicine and reducing implementation costs. Here, we report on the development of a novel SMDC profiling tool to examine populations of cells in vitro derived from different donors. We developed an image-based pipeline to quantify morphological features and extracted cell shape descriptors. We investigated whether these could predict heterogeneity in the formation of myotubes and correlate with the myogenic fusion index. Several of the early cell shape characteristics were found to negatively correlate with the fusion index. These included total area occupied by cells, area shape, bounding box area, compactness, equivalent diameter, minimum ferret diameter, minor axis length and perimeter of SMDC at 24 h after initiating culture. The information extracted with our approach indicates live cell imaging can detect a range of cell phenotypes based on cell-shape alone and preserving cell integrity could be used to predict propensity to form myotubes in vitro and functional tissue in vivo.<\/p>\n\n\n\n

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