{"id":31497,"date":"2025-04-15T15:27:46","date_gmt":"2025-04-15T15:27:46","guid":{"rendered":"https:\/\/amelie-project.eu\/?post_type=publication&p=31497"},"modified":"2025-12-17T16:46:58","modified_gmt":"2025-12-17T16:46:58","slug":"las-microesferas-visibles-en-ct-permiten-el-seguimiento-in-vivo-de-todo-el-cuerpo-de-andamiajes-inyectables-de-ingenieria-tisular","status":"publish","type":"publication","link":"https:\/\/amelie-project.eu\/es\/publicacion\/las-microesferas-visibles-en-ct-permiten-el-seguimiento-in-vivo-de-todo-el-cuerpo-de-andamiajes-inyectables-de-ingenieria-tisular\/","title":{"rendered":"Las microesferas visibles por tomograf\u00eda computarizada permiten el seguimiento in vivo de todo el cuerpo de los andamios inyectables de ingenier\u00eda tisular"},"content":{"rendered":"

[et_pb_section fb_built=”1″ admin_label=”section” _builder_version=”4.16″ global_colors_info=”{}”][et_pb_row admin_label=”row” _builder_version=”4.21.0″ background_size=”initial” background_position=”top_left” background_repeat=”repeat” width=”100%” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_text admin_label=”Text” _builder_version=”4.21.0″ background_size=”initial” background_position=”top_left” background_repeat=”repeat” global_colors_info=”{}”]<\/p>\n

Annalisa Bettini, Peter Stephen Patrick, Richard M. Day, Daniel J. Stuckey.<\/strong><\/p>\n

Materiales sanitarios avanzados. 2024;13(17).<\/p>\n

Resumen<\/em><\/p>\n

En este estudio se han desarrollado microesferas diminutas (llamadas microportadores) que pueden transportar c\u00e9lulas terap\u00e9uticas y, lo que es m\u00e1s importante, pueden verse claramente en las exploraciones m\u00e9dicas por imagen. Los investigadores a\u00f1adieron un material de contraste inocuo, el sulfato de bario, para que las microesferas aparecieran en las tomograf\u00edas computarizadas (TC) tras ser inyectadas en el cuerpo. De este modo, los m\u00e9dicos pueden saber a d\u00f3nde va el material implantado, cu\u00e1nto tiempo permanece en su sitio y si las c\u00e9lulas trasplantadas siguen vivas. En pruebas de laboratorio y estudios con animales, las microesferas resultaron seguras, permitieron el crecimiento de c\u00e9lulas y permanecieron visibles en los esc\u00e1neres durante al menos dos semanas. Las microesferas tambi\u00e9n podr\u00edan administrarse mediante inyecciones m\u00ednimamente invasivas, incluso en el coraz\u00f3n. En general, esta tecnolog\u00eda podr\u00eda ayudar a mejorar las terapias regenerativas al garantizar que los andamiajes celulares implantados lleguen al lugar correcto y permanezcan all\u00ed, al tiempo que ofrece a los m\u00e9dicos una forma de supervisar el tratamiento en tiempo real.<\/p>\n

Resumen<\/em><\/p>\n

La administraci\u00f3n y retenci\u00f3n selectivas son requisitos esenciales de los productos implantables de ingenier\u00eda tisular. Los m\u00e9todos no invasivos de obtenci\u00f3n de im\u00e1genes que puedan confirmar la localizaci\u00f3n, retenci\u00f3n y biodistribuci\u00f3n de c\u00e9lulas trasplantadas adheridas a andamiajes de ingenier\u00eda tisular implantados ser\u00e1n de gran valor para la optimizaci\u00f3n y mejora de las terapias regenerativas. Para dar respuesta a esta necesidad, se modifica un andamiaje inyectable de ingenier\u00eda tisular compuesto por microesferas altamente porosas compatibles con el trasplante de c\u00e9lulas para que contengan el agente de contraste para tomograf\u00eda computarizada (TC) sulfato de bario (BaSO4). Las microesferas rastreables muestran una elevada absorci\u00f3n de rayos X, con un contraste que permite el rastreo de todo el cuerpo. Las microesferas se han celularizado con c\u00e9lulas madre mesenquimales GFP+ Luciferase+ y muestran biocompatibilidad in vitro. In vivo, las microesferas celularizadas cargadas con BaSO4 se administran en la extremidad posterior de ratones, donde permanecen viables durante 14 d\u00edas. El registro conjunto de im\u00e1genes 3D bioluminiscentes y reconstrucciones \u00b5CT permite evaluar el material del andamio y la co-localizaci\u00f3n celular. Las microesferas rastreables tambi\u00e9n son compatibles con la administraci\u00f3n m\u00ednimamente invasiva mediante inyecciones intramioc\u00e1rdicas transtor\u00e1cicas guiadas por ultrasonidos en ratas. Estos resultados sugieren que las microesferas cargadas con BaSO4 pueden utilizarse como una herramienta novedosa para optimizar las t\u00e9cnicas de administraci\u00f3n y rastrear la persistencia y distribuci\u00f3n de los materiales de andamiaje implantados. Adem\u00e1s, las microesferas pueden ser celularizadas y tienen potencial para convertirse en un producto combinado inyectable de ingenier\u00eda tisular para la regeneraci\u00f3n cardiaca.<\/span><\/p>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n

<\/p>\n

Acceda al documento completo aqu\u00ed:<\/p>\n

https:\/\/advanced.onlinelibrary.wiley.com\/doi\/full\/10.1002\/adhm.202303588<\/a>\u00a0<\/p>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n

[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section]<\/p>","protected":false},"excerpt":{"rendered":"

Annalisa Bettini, Peter Stephen Patrick, Richard M. Day, Daniel J. Stuckey. Advanced Healthcare Materials. 2024;13(17). Summary This study developed tiny sponge-like beads (called microcarriers) that can carry therapeutic cells and, importantly, can be seen clearly on medical imaging scans. The researchers added a harmless contrast material, barium sulphate, so the beads show up on computed tomography (CT) scans after they are injected into the body. This makes it possible for doctors to track where the implanted material goes, how long it stays in place, and whether the transplanted cells remain alive. In laboratory tests and in animal studies, the beads were safe, allowed cells to grow on them, and stayed visible on scans for at least two weeks. The microspheres could also be delivered through minimally invasive injections, including into the heart. Overall, this technology could help improve regenerative therapies by ensuring that implanted cell-carrying scaffolds reach the right location and stay there \u2013 while giving doctors a way to monitor the treatment in real time. Abstract Targeted delivery and retention are essential requirements for implantable tissue-engineered products. Non-invasive imaging methods that can confirm location, retention, and biodistribution of transplanted cells attached to implanted tissue engineering scaffolds will be invaluable […]<\/p>","protected":false},"featured_media":31499,"template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"\n

Charlotte Desprez, Davide Danovi, Charles H Knowles and Richard M Day.<\/p>\n\n\n\n

J. Tissue Eng. 2023;14:1\u201318.<\/p>\n\n\n\n

Abstract<\/em><\/p>\n\n\n\n

Skeletal muscle-derived cells (SMDC) hold tremendous potential for replenishing dysfunctional muscle lost due to disease or trauma. Current therapeutic usage of SMDC relies on harvesting autologous cells from muscle biopsies that are subsequently expanded in vitro before re-implantation into the patient. Heterogeneity can arise from multiple factors including quality of the starting biopsy, age and comorbidity affecting the processed SMDC. Quality attributes intended for clinical use often focus on minimum levels of myogenic cell marker expression. Such approaches do not evaluate the likelihood of SMDC to differentiate and form myofibres when implanted in vivo, which ultimately determines the likelihood of muscle regeneration. Predicting the therapeutic potency of SMDC in vitro prior to implantation is key to developing successful therapeutics in regenerative medicine and reducing implementation costs. Here, we report on the development of a novel SMDC profiling tool to examine populations of cells in vitro derived from different donors. We developed an image-based pipeline to quantify morphological features and extracted cell shape descriptors. We investigated whether these could predict heterogeneity in the formation of myotubes and correlate with the myogenic fusion index. Several of the early cell shape characteristics were found to negatively correlate with the fusion index. These included total area occupied by cells, area shape, bounding box area, compactness, equivalent diameter, minimum ferret diameter, minor axis length and perimeter of SMDC at 24 h after initiating culture. The information extracted with our approach indicates live cell imaging can detect a range of cell phenotypes based on cell-shape alone and preserving cell integrity could be used to predict propensity to form myotubes in vitro and functional tissue in vivo.<\/p>\n\n\n\n

Access the full paper here: https:\/\/pubmed.ncbi.nlm.nih.gov\/36949843\/<\/a><\/p>\n","_et_gb_content_width":"","_coblocks_attr":"","_coblocks_dimensions":"","_coblocks_responsive_height":"","_coblocks_accordion_ie_support":"","_links_to":"","_links_to_target":""},"categories":[43],"class_list":["post-31497","publication","type-publication","status-publish","has-post-thumbnail","hentry","category-publication"],"_links":{"self":[{"href":"https:\/\/amelie-project.eu\/es\/wp-json\/wp\/v2\/publication\/31497","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/amelie-project.eu\/es\/wp-json\/wp\/v2\/publication"}],"about":[{"href":"https:\/\/amelie-project.eu\/es\/wp-json\/wp\/v2\/types\/publication"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/amelie-project.eu\/es\/wp-json\/wp\/v2\/media\/31499"}],"wp:attachment":[{"href":"https:\/\/amelie-project.eu\/es\/wp-json\/wp\/v2\/media?parent=31497"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/amelie-project.eu\/es\/wp-json\/wp\/v2\/categories?post=31497"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}