{"id":29993,"date":"2023-12-29T09:09:34","date_gmt":"2023-12-29T09:09:34","guid":{"rendered":"https:\/\/amelie-project.eu\/?post_type=category-publication&p=29993"},"modified":"2024-02-26T16:21:43","modified_gmt":"2024-02-26T16:21:43","slug":"publicacion-2","status":"publish","type":"publication","link":"https:\/\/amelie-project.eu\/es\/publicacion\/publicacion-2\/","title":{"rendered":"Desarrollo de un producto de ingenier\u00eda tisular microportador celular para la reparaci\u00f3n muscular mediante un sistema de biorreactor"},"content":{"rendered":"[et_pb_section fb_built=”1″ admin_label=”section” _builder_version=”4.16″ global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_row admin_label=”row” _builder_version=”4.21.0″ background_size=”initial” background_position=”top_left” background_repeat=”repeat” hover_enabled=”0″ global_colors_info=”{}” theme_builder_area=”post_content” width=”100%” sticky_enabled=”0″][et_pb_column type=”4_4″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||” theme_builder_area=”post_content”][et_pb_text admin_label=”Text” _builder_version=”4.21.0″ background_size=”initial” background_position=”top_left” background_repeat=”repeat” hover_enabled=”0″ global_colors_info=”{}” theme_builder_area=”post_content” sticky_enabled=”0″]
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Ana Lu\u00edsa Cartaxo, Ana Fernandes-Platzgummer, Carlos A V Rodrigues, Ana M Melo, Katja Tecklenburg, Eva Margreiter, Richard M Day, Cl\u00e1udia L da Silva y Joaquim M S Cabral.<\/strong>
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Tissue Eng Part C Methods. 2023;29(12):583-595.<\/p>\n

La incontinencia fecal, aunque no pone en peligro la vida, tiene un gran impacto en la econom\u00eda y en la calidad de vida del paciente. Hasta ahora, los tratamientos disponibles se basan en enfoques quir\u00fargicos y no quir\u00fargicos. Pueden ir desde cambios en la dieta hasta entrenamiento intestinal o estimulaci\u00f3n del nervio sacro, pero ninguno de ellos ofrece una soluci\u00f3n a largo plazo. Est\u00e1n surgiendo nuevas terapias basadas en la medicina regenerativa, cuyo objetivo es regenerar el m\u00fasculo del esf\u00ednter y restablecer la continencia. Suelen consistir en la administraci\u00f3n de una suspensi\u00f3n de c\u00e9lulas musculares esquel\u00e9ticas expandidas (SkMDC) en la zona da\u00f1ada. Sin embargo, esta estrategia suele dar lugar a una viabilidad celular reducida debido a la necesidad de recoger las c\u00e9lulas de la plataforma de expansi\u00f3n, as\u00ed como al uso no nativo de una suspensi\u00f3n celular para administrar las c\u00e9lulas dependientes de anclaje. En este estudio, proponemos la prueba de concepto para el bioprocesamiento de un nuevo m\u00e9todo de administraci\u00f3n celular para el tratamiento de la incontinencia fecal, obtenido mediante un proceso escalable en dos pasos. En primer lugar, se expandieron SkMDC aisladas de pacientes utilizando sistemas de cultivo est\u00e1ticos planares. En segundo lugar, utilizando un biorreactor PBS-MINI Vertical-Wheel\u00ae de un solo uso, los SkMDC expandidos se combinaron con microportadores de poli(\u00e1cido l\u00e1ctico-co-glic\u00f3lico) biocompatibles y biodegradables (es decir, directamente implantables) preparados mediante separaci\u00f3n de fases inducida t\u00e9rmicamente. Este proceso permiti\u00f3 que las SkMDC se adhirieran a los microportadores con una eficacia de hasta 80%. Es importante destacar que los SkMDC fueron viables durante todo el proceso y mantuvieron sus caracter\u00edsticas miog\u00e9nicas (por ejemplo, la expresi\u00f3n del marcador CD56) tras la adhesi\u00f3n y el cultivo en los microportadores. Cuando los microportadores que conten\u00edan SkMDC se colocaron en una placa de cultivo, las c\u00e9lulas fueron capaces de migrar desde los microportadores a la superficie de cultivo y diferenciarse en miotubos multinucleados, lo que pone de manifiesto su potencial para regenerar el m\u00fasculo del esf\u00ednter da\u00f1ado tras su administraci\u00f3n al paciente. En general, este estudio propone un m\u00e9todo innovador para fijar SkMDC a microportadores biodegradables, lo que puede proporcionar un nuevo tratamiento para la incontinencia fecal.<\/p>\n

Acceda al documento completo aqu\u00ed:\u00a0<\/span><\/p>\n

https:\/\/pubmed.ncbi.nlm.nih.gov\/37842845\/<\/a><\/p>\n<\/div>\n<\/div>\n

<\/p>[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section]","protected":false},"excerpt":{"rendered":"

Ana Lu\u00edsa Cartaxo, Ana Fernandes-Platzgummer, Carlos A V Rodrigues, Ana M Melo, Katja Tecklenburg, Eva Margreiter, Richard M Day, Cl\u00e1udia L da Silva, and Joaquim M S Cabral. Tissue Eng Part C Methods. 2023;29(12):583\u2013595. Fecal incontinence, although not life-threatening, has a high impact on the economy and patient quality of life. So far, available treatments are based on both surgical and nonsurgical approaches. These can range from changes in diet, to bowel training, or sacral nerve stimulation, but none of which provides a long-term solution. New regenerative medicine-based therapies are emerging, which aim at regenerating the sphincter muscle and restoring continence. Usually, these consist of the administration of a suspension of expanded skeletal-derived muscle cells (SkMDCs) to the damaged site. However, this strategy often results in a reduced cell viability due to the need for cell harvesting from the expansion platform, as well as the non-native use of a cell suspension to deliver the anchorage-dependent cells. In this study, we propose the proof-of-concept for the bioprocessing of a new cell delivery method for the treatment of fecal incontinence, obtained by a scalable two-step process. First, patient-isolated SkMDCs were expanded using planar static culture systems. Second, by using a single-use PBS-MINI […]<\/p>","protected":false},"featured_media":31458,"template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"\n

Lorem Ipsum<\/strong> is simply dummy text of the printing and typesetting industry. Lorem Ipsum has been the industry's standard dummy text ever since the 1500s, when an unknown printer took a galley of type and scrambled it to make a type specimen book. It has survived not only five centuries, but also the leap into electronic typesetting, remaining essentially unchanged. It was popularised in the 1960s with the release of Letraset sheets containing Lorem Ipsum passages, and more recently with desktop publishing software like Aldus PageMaker including versions of Lorem Ipsum.<\/p>\n","_et_gb_content_width":"","_coblocks_attr":"","_coblocks_dimensions":"","_coblocks_responsive_height":"","_coblocks_accordion_ie_support":"","_links_to":"","_links_to_target":""},"categories":[43],"class_list":["post-29993","publication","type-publication","status-publish","has-post-thumbnail","hentry","category-publication"],"_links":{"self":[{"href":"https:\/\/amelie-project.eu\/es\/wp-json\/wp\/v2\/publication\/29993","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/amelie-project.eu\/es\/wp-json\/wp\/v2\/publication"}],"about":[{"href":"https:\/\/amelie-project.eu\/es\/wp-json\/wp\/v2\/types\/publication"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/amelie-project.eu\/es\/wp-json\/wp\/v2\/media\/31458"}],"wp:attachment":[{"href":"https:\/\/amelie-project.eu\/es\/wp-json\/wp\/v2\/media?parent=29993"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/amelie-project.eu\/es\/wp-json\/wp\/v2\/categories?post=29993"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}