{"id":29992,"date":"2023-01-14T09:09:30","date_gmt":"2023-01-14T09:09:30","guid":{"rendered":"https:\/\/amelie-project.eu\/?post_type=category-publication&p=29992"},"modified":"2024-02-26T16:21:23","modified_gmt":"2024-02-26T16:21:23","slug":"publicacion-1","status":"publish","type":"publication","link":"https:\/\/amelie-project.eu\/es\/publicacion\/publicacion-1\/","title":{"rendered":"Caracter\u00edsticas de la forma celular de las c\u00e9lulas musculares esquel\u00e9ticas humanas como predictor de la competencia miog\u00e9nica: Un nuevo paradigma hacia la terapia celular de precisi\u00f3n"},"content":{"rendered":"[et_pb_section fb_built=”1″ admin_label=”section” _builder_version=”4.16″ global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_row admin_label=”row” _builder_version=”4.21.0″ background_size=”initial” background_position=”top_left” background_repeat=”repeat” hover_enabled=”0″ global_colors_info=”{}” theme_builder_area=”post_content” width=”100%” sticky_enabled=”0″][et_pb_column type=”4_4″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||” theme_builder_area=”post_content”][et_pb_text admin_label=”Text” _builder_version=”4.21.0″ background_size=”initial” background_position=”top_left” background_repeat=”repeat” hover_enabled=”0″ global_colors_info=”{}” theme_builder_area=”post_content” sticky_enabled=”0″]

Charlotte Desprez, Davide Danovi, Charles H Knowles y Richard M Day.<\/strong><\/p>\n

J. Tissue Eng. 2023;14:1-18.<\/p>\n

Resumen<\/em><\/p>\n

Las c\u00e9lulas derivadas del m\u00fasculo esquel\u00e9tico (CMME) encierran un enorme potencial para reponer el m\u00fasculo disfuncional perdido a causa de una enfermedad o un traumatismo. El uso terap\u00e9utico actual de las SMDC se basa en la obtenci\u00f3n de c\u00e9lulas aut\u00f3logas a partir de biopsias musculares que posteriormente se expanden in vitro antes de reimplantarlas en el paciente. La heterogeneidad puede deberse a m\u00faltiples factores, como la calidad de la biopsia inicial, la edad y la comorbilidad que afectan a las SMDC procesadas. Los atributos de calidad destinados al uso cl\u00ednico suelen centrarse en niveles m\u00ednimos de expresi\u00f3n de marcadores celulares miog\u00e9nicos. Estos enfoques no eval\u00faan la probabilidad de que las SMDC se diferencien y formen miofibras cuando se implantan in vivo, lo que en \u00faltima instancia determina la probabilidad de regeneraci\u00f3n muscular. Predecir la potencia terap\u00e9utica de los SMDC in vitro antes de su implantaci\u00f3n es clave para desarrollar terapias de \u00e9xito en medicina regenerativa y reducir los costes de implantaci\u00f3n. En este art\u00edculo presentamos el desarrollo de una novedosa herramienta de perfilado de SMDC para examinar in vitro poblaciones de c\u00e9lulas derivadas de diferentes donantes. Desarrollamos un proceso basado en im\u00e1genes para cuantificar las caracter\u00edsticas morfol\u00f3gicas y extraer descriptores de la forma celular. Se investig\u00f3 si pod\u00edan predecir la heterogeneidad en la formaci\u00f3n de miotubos y correlacionarse con el \u00edndice de fusi\u00f3n miog\u00e9nica. Varias de las primeras caracter\u00edsticas de la forma celular mostraron una correlaci\u00f3n negativa con el \u00edndice de fusi\u00f3n. \u00c9stas inclu\u00edan el \u00e1rea total ocupada por las c\u00e9lulas, la forma del \u00e1rea, el \u00e1rea de la caja delimitadora, la compacidad, el di\u00e1metro equivalente, el di\u00e1metro m\u00ednimo del hur\u00f3n, la longitud del eje menor y el per\u00edmetro del SMDC a las 24 h de iniciar el cultivo. La informaci\u00f3n extra\u00edda con nuestro enfoque indica que las im\u00e1genes de c\u00e9lulas vivas pueden detectar una serie de fenotipos celulares bas\u00e1ndose \u00fanicamente en la forma celular y que la preservaci\u00f3n de la integridad celular podr\u00eda utilizarse para predecir la propensi\u00f3n a formar miotubos in vitro y tejido funcional in vivo.<\/p>\n

<\/p>\n

Acceda al documento completo aqu\u00ed:<\/p>\n

https:\/\/pubmed.ncbi.nlm.nih.gov\/36949843\/<\/a><\/p>\n

<\/p>[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section]","protected":false},"excerpt":{"rendered":"

Charlotte Desprez, Davide Danovi, Charles H Knowles and Richard M Day. J. Tissue Eng. 2023;14:1\u201318. Abstract Skeletal muscle-derived cells (SMDC) hold tremendous potential for replenishing dysfunctional muscle lost due to disease or trauma. Current therapeutic usage of SMDC relies on harvesting autologous cells from muscle biopsies that are subsequently expanded in vitro before re-implantation into the patient. Heterogeneity can arise from multiple factors including quality of the starting biopsy, age and comorbidity affecting the processed SMDC. Quality attributes intended for clinical use often focus on minimum levels of myogenic cell marker expression. Such approaches do not evaluate the likelihood of SMDC to differentiate and form myofibres when implanted in vivo, which ultimately determines the likelihood of muscle regeneration. Predicting the therapeutic potency of SMDC in vitro prior to implantation is key to developing successful therapeutics in regenerative medicine and reducing implementation costs. Here, we report on the development of a novel SMDC profiling tool to examine populations of cells in vitro derived from different donors. We developed an image-based pipeline to quantify morphological features and extracted cell shape descriptors. We investigated whether these could predict heterogeneity in the formation of myotubes and correlate with the myogenic fusion index. Several of […]<\/p>","protected":false},"featured_media":31457,"template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"\n

Charlotte Desprez, Davide Danovi, Charles H Knowles and Richard M Day.<\/p>\n\n\n\n

J. Tissue Eng. 2023;14:1\u201318.<\/p>\n\n\n\n

Abstract<\/em><\/p>\n\n\n\n

Skeletal muscle-derived cells (SMDC) hold tremendous potential for replenishing dysfunctional muscle lost due to disease or trauma. Current therapeutic usage of SMDC relies on harvesting autologous cells from muscle biopsies that are subsequently expanded in vitro before re-implantation into the patient. Heterogeneity can arise from multiple factors including quality of the starting biopsy, age and comorbidity affecting the processed SMDC. Quality attributes intended for clinical use often focus on minimum levels of myogenic cell marker expression. Such approaches do not evaluate the likelihood of SMDC to differentiate and form myofibres when implanted in vivo, which ultimately determines the likelihood of muscle regeneration. Predicting the therapeutic potency of SMDC in vitro prior to implantation is key to developing successful therapeutics in regenerative medicine and reducing implementation costs. Here, we report on the development of a novel SMDC profiling tool to examine populations of cells in vitro derived from different donors. We developed an image-based pipeline to quantify morphological features and extracted cell shape descriptors. We investigated whether these could predict heterogeneity in the formation of myotubes and correlate with the myogenic fusion index. Several of the early cell shape characteristics were found to negatively correlate with the fusion index. These included total area occupied by cells, area shape, bounding box area, compactness, equivalent diameter, minimum ferret diameter, minor axis length and perimeter of SMDC at 24 h after initiating culture. The information extracted with our approach indicates live cell imaging can detect a range of cell phenotypes based on cell-shape alone and preserving cell integrity could be used to predict propensity to form myotubes in vitro and functional tissue in vivo.<\/p>\n\n\n\n

Access the full paper here: https:\/\/pubmed.ncbi.nlm.nih.gov\/36949843\/<\/a><\/p>\n","_et_gb_content_width":"","_coblocks_attr":"","_coblocks_dimensions":"","_coblocks_responsive_height":"","_coblocks_accordion_ie_support":"","_links_to":"","_links_to_target":""},"categories":[43],"class_list":["post-29992","publication","type-publication","status-publish","has-post-thumbnail","hentry","category-publication"],"_links":{"self":[{"href":"https:\/\/amelie-project.eu\/es\/wp-json\/wp\/v2\/publication\/29992","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/amelie-project.eu\/es\/wp-json\/wp\/v2\/publication"}],"about":[{"href":"https:\/\/amelie-project.eu\/es\/wp-json\/wp\/v2\/types\/publication"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/amelie-project.eu\/es\/wp-json\/wp\/v2\/media\/31457"}],"wp:attachment":[{"href":"https:\/\/amelie-project.eu\/es\/wp-json\/wp\/v2\/media?parent=29992"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/amelie-project.eu\/es\/wp-json\/wp\/v2\/categories?post=29992"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}