{"id":29992,"date":"2023-01-14T09:09:30","date_gmt":"2023-01-14T09:09:30","guid":{"rendered":"https:\/\/amelie-project.eu\/?post_type=category-publication&p=29992"},"modified":"2024-02-26T16:21:23","modified_gmt":"2024-02-26T16:21:23","slug":"veroffentlichung-1","status":"publish","type":"publication","link":"https:\/\/amelie-project.eu\/de\/veroffentlichung\/veroffentlichung-1\/","title":{"rendered":"Zellformmerkmale menschlicher Skelettmuskelzellen als Pr\u00e4diktor f\u00fcr die myogene Kompetenz: Ein neues Paradigma f\u00fcr eine pr\u00e4zise Zelltherapie"},"content":{"rendered":"[et_pb_section fb_built=”1″ admin_label=”section” _builder_version=”4.16″ global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_row admin_label=”row” _builder_version=”4.21.0″ background_size=”initial” background_position=”top_left” background_repeat=”repeat” hover_enabled=”0″ global_colors_info=”{}” theme_builder_area=”post_content” width=”100%” sticky_enabled=”0″][et_pb_column type=”4_4″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||” theme_builder_area=”post_content”][et_pb_text admin_label=”Text” _builder_version=”4.21.0″ background_size=”initial” background_position=”top_left” background_repeat=”repeat” hover_enabled=”0″ global_colors_info=”{}” theme_builder_area=”post_content” sticky_enabled=”0″]

Charlotte Desprez, Davide Danovi, Charles H. Knowles und Richard M. Day.<\/strong><\/p>\n

J. Tissue Eng. 2023;14:1-18.<\/p>\n

Abstrakt<\/em><\/p>\n

Skelettmuskelzellen (SMDC) bergen ein enormes Potenzial f\u00fcr die Wiederherstellung funktionsgest\u00f6rter Muskeln, die aufgrund von Krankheiten oder Traumata verloren gegangen sind. Die derzeitige therapeutische Verwendung von SMDC beruht auf der Entnahme autologer Zellen aus Muskelbiopsien, die anschlie\u00dfend in vitro expandiert werden, bevor sie dem Patienten wieder implantiert werden. Heterogenit\u00e4t kann durch verschiedene Faktoren entstehen, darunter die Qualit\u00e4t der Ausgangsbiopsie, das Alter und Begleiterkrankungen, die sich auf das verarbeitete SMDC auswirken. Qualit\u00e4tsmerkmale f\u00fcr den klinischen Einsatz konzentrieren sich h\u00e4ufig auf ein Mindestma\u00df an myogener Zellmarkerexpression. Bei solchen Ans\u00e4tzen wird nicht bewertet, wie wahrscheinlich es ist, dass SMDC sich differenzieren und Myofasern bilden, wenn sie in vivo implantiert werden, was letztlich \u00fcber die Wahrscheinlichkeit der Muskelregeneration entscheidet. Die Vorhersage der therapeutischen Wirksamkeit von SMDC in vitro vor der Implantation ist der Schl\u00fcssel zur Entwicklung erfolgreicher Therapeutika in der regenerativen Medizin und zur Reduzierung der Implementierungskosten. Hier berichten wir \u00fcber die Entwicklung eines neuartigen SMDC-Profiling-Tools zur Untersuchung von Zellpopulationen in vitro, die von verschiedenen Spendern stammen. Wir entwickelten eine bildbasierte Pipeline zur Quantifizierung morphologischer Merkmale und extrahierten Deskriptoren der Zellform. Wir untersuchten, ob diese die Heterogenit\u00e4t bei der Bildung von Myotubes vorhersagen und mit dem myogenen Fusionsindex korrelieren k\u00f6nnen. Es zeigte sich, dass mehrere der fr\u00fchen Zellformmerkmale negativ mit dem Fusionsindex korrelieren. Dazu geh\u00f6rten die von den Zellen eingenommene Gesamtfl\u00e4che, die Fl\u00e4chenform, die Bounding-Box-Fl\u00e4che, die Kompaktheit, der \u00e4quivalente Durchmesser, der minimale Ferret-Durchmesser, die L\u00e4nge der Nebenachse und der Umfang der SMDC 24 Stunden nach Beginn der Kultur. Die mit unserem Ansatz gewonnenen Informationen zeigen, dass die Bildgebung von lebenden Zellen eine Reihe von Zellph\u00e4notypen allein auf der Grundlage der Zellform erkennen kann und dass die Erhaltung der Zellintegrit\u00e4t zur Vorhersage der Neigung zur Bildung von Myotubes in vitro und von funktionellem Gewebe in vivo genutzt werden k\u00f6nnte.<\/p>\n

<\/p>\n

Hier finden Sie das vollst\u00e4ndige Papier:<\/p>\n

https:\/\/pubmed.ncbi.nlm.nih.gov\/36949843\/<\/a><\/p>\n

<\/p>[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section]","protected":false},"excerpt":{"rendered":"

Charlotte Desprez, Davide Danovi, Charles H Knowles and Richard M Day. J. Tissue Eng. 2023;14:1\u201318. Abstract Skeletal muscle-derived cells (SMDC) hold tremendous potential for replenishing dysfunctional muscle lost due to disease or trauma. Current therapeutic usage of SMDC relies on harvesting autologous cells from muscle biopsies that are subsequently expanded in vitro before re-implantation into the patient. Heterogeneity can arise from multiple factors including quality of the starting biopsy, age and comorbidity affecting the processed SMDC. Quality attributes intended for clinical use often focus on minimum levels of myogenic cell marker expression. Such approaches do not evaluate the likelihood of SMDC to differentiate and form myofibres when implanted in vivo, which ultimately determines the likelihood of muscle regeneration. Predicting the therapeutic potency of SMDC in vitro prior to implantation is key to developing successful therapeutics in regenerative medicine and reducing implementation costs. Here, we report on the development of a novel SMDC profiling tool to examine populations of cells in vitro derived from different donors. We developed an image-based pipeline to quantify morphological features and extracted cell shape descriptors. We investigated whether these could predict heterogeneity in the formation of myotubes and correlate with the myogenic fusion index. Several of […]<\/p>","protected":false},"featured_media":31457,"template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"\n

Charlotte Desprez, Davide Danovi, Charles H Knowles and Richard M Day.<\/p>\n\n\n\n

J. Tissue Eng. 2023;14:1\u201318.<\/p>\n\n\n\n

Abstract<\/em><\/p>\n\n\n\n

Skeletal muscle-derived cells (SMDC) hold tremendous potential for replenishing dysfunctional muscle lost due to disease or trauma. Current therapeutic usage of SMDC relies on harvesting autologous cells from muscle biopsies that are subsequently expanded in vitro before re-implantation into the patient. Heterogeneity can arise from multiple factors including quality of the starting biopsy, age and comorbidity affecting the processed SMDC. Quality attributes intended for clinical use often focus on minimum levels of myogenic cell marker expression. Such approaches do not evaluate the likelihood of SMDC to differentiate and form myofibres when implanted in vivo, which ultimately determines the likelihood of muscle regeneration. Predicting the therapeutic potency of SMDC in vitro prior to implantation is key to developing successful therapeutics in regenerative medicine and reducing implementation costs. Here, we report on the development of a novel SMDC profiling tool to examine populations of cells in vitro derived from different donors. We developed an image-based pipeline to quantify morphological features and extracted cell shape descriptors. We investigated whether these could predict heterogeneity in the formation of myotubes and correlate with the myogenic fusion index. Several of the early cell shape characteristics were found to negatively correlate with the fusion index. These included total area occupied by cells, area shape, bounding box area, compactness, equivalent diameter, minimum ferret diameter, minor axis length and perimeter of SMDC at 24 h after initiating culture. The information extracted with our approach indicates live cell imaging can detect a range of cell phenotypes based on cell-shape alone and preserving cell integrity could be used to predict propensity to form myotubes in vitro and functional tissue in vivo.<\/p>\n\n\n\n

Access the full paper here: https:\/\/pubmed.ncbi.nlm.nih.gov\/36949843\/<\/a><\/p>\n","_et_gb_content_width":"","_coblocks_attr":"","_coblocks_dimensions":"","_coblocks_responsive_height":"","_coblocks_accordion_ie_support":"","_links_to":"","_links_to_target":""},"categories":[43],"class_list":["post-29992","publication","type-publication","status-publish","has-post-thumbnail","hentry","category-publication"],"_links":{"self":[{"href":"https:\/\/amelie-project.eu\/de\/wp-json\/wp\/v2\/publication\/29992","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/amelie-project.eu\/de\/wp-json\/wp\/v2\/publication"}],"about":[{"href":"https:\/\/amelie-project.eu\/de\/wp-json\/wp\/v2\/types\/publication"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/amelie-project.eu\/de\/wp-json\/wp\/v2\/media\/31457"}],"wp:attachment":[{"href":"https:\/\/amelie-project.eu\/de\/wp-json\/wp\/v2\/media?parent=29992"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/amelie-project.eu\/de\/wp-json\/wp\/v2\/categories?post=29992"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}